trinity assembler manual

Lets build the Trinity synth!!! Before you dress up your trinity is really good idea to test that everything works (We'll put a debugging tutorial online soon). Rotate your device and follow the step-by-step assembly instructions. View All Hoods, Free-standing Users must provide read alignments to Trinity as a coordinate-sorted bam file. 16Mhz resonator has no polarity. Turn clockwise to screw in place. THIS MANUAL IS ONLY FOR PROFESSIONAL BICYCLE MECHANICS AT AUTHORIZED GIANT DEALERS. That means three beings in one - God the Father, God the Son and God the Holy Spirit - a 'triune' God. On board v1.0 there are 3 long spacers of the same length and one longer which compensates the height difference. View All Dishwashers, Free-standing There are no questions too small, or any problems too big. TRINITY model numbers usually start with: TBF, THA, TLS, TSN, & TXK and are then followed by a 4-5 digit number. This script is not sending in sbatch mode because it could be take time in this training. put the battery inside and flip the switch to battery position, plug an adapter and flip the switch to plug position. No tools required for assembly. Under Counter NCBI SRA library. TRINITY ADVANCED PRO Instruction manual Giant TRINITY ADVANCED PRO Instruction Manual TRINITY ADVANCED PRO Instruction manual ATTENTION! You can clearly see it on the images. The Company assumes that this material is utilized by the End User. SERVICE PARTS LIST TBFPGR-2408 / TBFPRA-2408, TRINITY Wire Shelving Rack Owners Manual, Trinity Pro 36 Locker Cabinet Owners Manual. Please read the instructions CAREFULLY especially for the power switch! You can find more informations at our website: bastl-instruments.com Before you start: The board solders from both sides. The new model of our ViolinCase has similar properties as our original model and can be combined with other parts of the Trinity Set, but there are some important changes and improvements. Use the silver plastic piece which holds the cable so it does not break. This collection consists of manuals, circuit diagrams, cheat sheets and other materials related to the operation of arcade machines. Trinity Trinity is a de novo assembler that fully reconstructs a large fraction of transcripts, including alternatively spliced isoforms and transcripts from recently duplicated genes using de Bruijn graphs. Shipping TBD Canada, Open by Appointment only. It adopts 2.4G frequency band; with long controlled distance; allow some helicopters to fly in the same area without any interference. SINK (1) A Trinity Transcript Quantification There are now several methods available for estimating transcript abundance in a genome-free manner, and these include alignment-based methods (aligning reads to the transcript assembly) and alignment-free methods (typically examining k-mer abundances in the reads and in the resulting assemblies). Voltage regulator 7805 has polarity indicated. We cover the syntax expected in source les, including notation for symbols, constants, and expressions; the directives that asunderstands; and of course how to invoke as. Cooking Set 2 The above warranty gives you specific legal rights, and you may have other rights which vary from state to state. It divides the data to many smaller de bruijn graphs, each representing the transcriptional complexity at a locus. I had read one of Matthew MacManes' papers awhile back suggesting 40 million reads was a good number for Trinity assemblies of most metazoans.However, I have 28 samples across 3 treatments that I want to use for DESeq and so, if I follow the advice at BioStars . Screw the bottom plate just right, so your battery holds nicely without too much tension. Mobile & Tablets. Use GSNAP, TopHat, STAR or other favorite RNA-Seq read alignment tool to generate the bam file, and be sure it's coordinate sorted by running 'samtools sort' on it. We would love to see what you guys can come up with! The following table lists the assembler instructions by type, and provides the number of the page where the instruction is described. The assembly should take about 25 min. TRINITY Customer ServiceTEL: 800.985.5506FAX: 310.347.4134EMAIL: [emailprotected], Assembly instructions Weight Bench Package content: No. Project Activity See All Activity > Categories Bio-Informatics License BSD License Follow Trinity RNA-Seq Assembly Trinity RNA-Seq Assembly Web Site Version 1.2 have switch with black handle, ready to be placed onto PCB on corresponding place (like on picture of fully assembled Trinity). If the option is not available, please select 'Other' and write it in the Comments field below. 8) Cautions regarding the use of the product are stated at the beginning and end of each owners manual. If the option is not available, please select 'Other' and write it in the Comments field below. You are done now! It depends on a number of factors including the number of reads to be assembled and the complexity of the transcript graphs. Howdy everyone! Choose the selection in the search results. Consumables (e.g. Read the board carefully! We accept no responsibility for any videos from third-party sources. Left and right knob are easy to place and solder. Did you make this project? Choose the selection in the search results. Trinity Assembly is a loving, friendly church where you can experience the life changing ministry of Jesus Christ and discover His will for your life. Undermount To help students understand something of the Christian doctrine of the Trinity. Duplication of contents of owners manuals and/or product literature in part or in whole without the permission of the Company for the purposes of sharing, mass-producing, posting online, or personal gain of any kind is prohibited by copyright law. It is also useful for users who would like to set-up and run Trinity for the first time. If you are missing any parts, need assistance with assembly or have questions, please contact TRINITY Customer Service: 800.985.5506 or [emailprotected]. Parts can also be requested online at www.trinityii.com (Help & More, Contact Us). 4-Axis Aerocraft Aerial Photography Drone 4-AXIS ABROCRAFT INSTRUCTION MANUAL This 4-axis aero craft is suitable for indoor/outdoor flying, but make sure the outdoor wind force is not more than grade 4. Lets build the Trinity synth!!! If there are any foils on the plastic remove them first. Slanted A downlink sends telemetry data from the Trinity to Q Base ground control and . Please inspect box contents to ensure you have received all components. Trinity RNA-Seq Assembler Performance Optimization Robert Henschel Indiana University 2709 E. Tenth Street Bloomington, IN 47401 Matthias Lieber Technische Universitt Dresden 01062 Dresden, Germany Le-Shin Wu Indiana University 2709 E. Tenth Street Bloomington, IN 47401 henschel@iu.edu Phillip M. Nista Indiana University 2709 E. Everything works? Please let us know if the video is no longer working. The Trinity RNA-Seq Assembly project provides software solutions targeted to the reconstruction of full-length transcripts and alternatively spliced isoforms from Illumina RNA-Seq data. Integrated Telephone +1-905-373-6571 Canada Eastern Standard Time. Information on korg.com may be updated as necessary based on changes to the product specification, operating system, included contents, and so on. Solderthe 6 pin bent header on the right place. Check if you can press easily all the buttons and tweak all the knobs - if not try to fix that by making them straight again. Larry Trey. OtherTRFSGO901TRFSECO600TRFSECO900TRFSEGO600TRFSEGO900TRFSEIO600TRFSEIO900TRFSGO601TRO605SSTRO600BKTRO600SSTRO606BKTRO606SSTRO900TRT6B31BKTRO6010BKTRO6010SSTROD600BKTROD600SSTROP600BKTROT600BKTRO605BKTRC600TRC600-EKTRAD770TRC603TRC604TRC605TRC775TRCI603TRI450TRI603TRI604TRI905TRC450TRG600BKTRG600SSTRG700BKTRG700SSTRG900BKTRG900SSTRC900CTTRG450TRI64-64N05TTR506B60TR506B90TR20160TR20190TR30160TR30190TR90660TR90690TR206A60TR206A90TR206B75TR206B90TR913B52TR913B90TR10860TR10890TR12990TR50275TR50290TR91260TR91290TR110120TR912B60BKTR912B90BKTR72360BKTR72390BKTR72360WTR72390WTR90960TR90990TRDWFS450TRDWFS600TRDWFS601TRDWFS4509TRDWFS6012TRDWFS6014TRTM210SSTRTM210WTRTM240SSTRTM240WTRTM400SSTRTM400WTRCF150WTRCF208WTRUF180WTRBC180TRWCD48TRWCS50TRCSG6010SSTRCSG6010BKTRCSC6010BKTRCSC6010SSTRMW600BKTRMW600SSTRMW23BKTRMW23SSTRWD25BKTRCCM180SS, Agree to privacy policy Remove the tape cover from the laser-cut plastic button covers and glue them into place. In order to register your product and receive streamlined customer service, please fill out the following Product Registration Form and (1) fax the form to 310.347.4134 (2) complete the Product Registration Form online at www.trinityii.com or (3) scan and email the form to [emailprotected]. Parts can also be requested online at www.trinityii.com(Help & More, Contact Us). Tip: if you manage to create some gap between header and PCB, just keep pushing the header and heat up the tin on a board. IMPORTANT:in version 1.1 with full plastic housing the switch has to go from the other side of the board! Than cut the remaining legs of the jack. on Introduction. Follow the polarity indicated by the notch of the socket. Butterfly (also called Parafly, referred to as Phase 2, assembling clusters of reads) In turn these 4 components can be grouped into two major phases: Phase 1: Clustering of RNA-Seq Reads: this is made up of the first three components. You will need no additional tools for assembly. The above video may be from a third-party source. Having trouble assemblying your TRINITY product? Required fields are marked *. Beverage Centre Functionally annotating transcripts using Trinotate and predicting coding regions using TransDecoder. Trinity, developed at the Broad Institute , represents a novel method for the efficient and robust de novo reconstruction of transcriptomes from RNA-Seq data. Mozzi library development kit has no casing and the small buttons are not tall. This is fixed on 1.1 board. To hold it in the place put something like pliers underneath, or solder first and last pins first, while pushing on header and finish all remaining connections afterward. Introduction to SPAdes genome assembler Andrew Severin His PhD was in Biophysics/NMR spectroscopy. Start typing to see products you are looking for. The pipeline starts off similar like any other sequencing w. Than cut the remaining pieces of buttons so the felt piece doesn't make a gap. Home TRINITY Trinity Shoe Bench Instruction Manual. TRINITY is a software package for conducting de novo (as well as the genome-guided version of) transcriptome assembly from RNA-seq data. TRINITY APPLIANCES PDF FILES ENQUIRY Your name* Last name Your email* Subject* Model Number * Found on the rating plate of your appliance. Then use screw and bolt to fix that in place (the last picture of this step). STEP 1: Screw FEET LEVELERS (E) into the bottoms of SIDE FRAME (A). View All Wine Coolers, Cooking Set 1 Because some of the holes had to be drilled bigger, therefore it might have happened that some of the connections here got lost. Your TRINITY Shoe Bench should include the following parts. Assembly. Installing Receiver and Magnetometer Do the 3 first and then longer one, which goes next to the power jack connector. On board version v1.0 there might be issues with the 3 LEDs. Trinity Sunday (15 June 2014) Suitable for Key Stage 4/5. You will need no additional tools for assembly. A Phillips head screwdriver is required for assembly, and a pipe wrench is recommended . If you are missing any parts, need assistance with assembly or have questions, please contact TRINITY Customer Service: 800.985.5506 or customerservice@trinityii.com. We can walk you through assembly process and provide more information. To troubleshoot individual graphs using Butterfly, see Advanced Guide to Trinity. We partnered with BILT to give you interactive, easy to follow 3D assembly & installation guides for your TRINITY items. The Company assumes that this material is utilized by the End User. phezuview. The first picture shows where everything goes. TotalView makes it easy. An informative whole school assembly about the significance of Trinity Sunday in the Christian calendar. Any warranty implied by applicable law is limited in duration to one year from the date of purchase and is subject to the same conditions and limitations as is provided for our express warranty. Screw FEET LEVELER (D) fully into each pre-installed nut on four corners of cabinet bottom. Cooking Set 3 Here, de novo assembly is restricted to only those reads that map to the genome. Here you can find Product Card , Technical Data Specification, Instruction Manual and more for your product. Share it with us! BILT is an all-in-one app that contains all information about your items including product registration and warranties. ASSEMBLY INSTRUCTIONS STEP 1 Lay CABINET BODY (A) gently on its back. Except as set forth herein, and to the extent of applicable there are no warranties on this Product either express or implied, and Trinity disclaims all warranties including, but not limited to, any implied warranties of merchantability, infringement or fitness for a particular purpose. Double check that you do solder from the correct side of the PCB! Email Sales. Trinity Foosball Cooler Important FOR QUICK & EASY 3D ASSEMBLY INSTRUCTIONS POUR DES DIRECTIVES D'ASSEMBLAGE 3D RAPIDES ET FACILES PARA INSTRUCCIONES DE ENSAMBLAJE RPIDAS Y SENCILLAS EN 3D PARTS LIST Your TRINITY Foosball Cooler should include the following parts. View online (68 pages) or download PDF (3 MB) Trinity Highway QuadGuard II User manual QuadGuard II PDF manual download and more Trinity Highway online manuals the authors of the trinity assembler also provide a package of programs called trinotate, which is designed to identify open reading frames in contigs, translate these to predicted polypeptide sequences, and characterize those polypeptides by comparison with public databases to produce an output file of annotation, but evaluation of transcriptome I wish to signup to the TRINITY Appliances newsletter for useful updates. The longer leg of the LED (anode) is marked on board as +. Documentation Take care of polarity and value of electrolytic capacitors the big ones. If your Product is defective or otherwise requires service or parts, please call TRINITY Customer Service toll-free at (800) 985-5506, between 5:00 a.m. and 5:00 p.m., PST. Users are permitted to create one (1) copy for their personal non-commercial use. -3x xOhm are 1K (brown black red)! Cellphones, GPS, Touch Pads, Bluetooth Headsets, etc. To place it onto PCB, carefully bend the legs of the slide switch into right angle, but take your time and do it ONE BY ONE! BILT Assembly Overviews After!downloading!the!data!file!'rnaseq_workshop_data.tgz',expandthecontents! It does not matter which side you bend them, but by not gentle bending legs, you might damage the switch. Header will end up as close as possible to PCB. If you accept the License Agreement, please download the software/file using the link below. One such denovo assembler, that we will showcase here is Trinity. Otherwise the PCB might not fit correctly into the housing!!! Combo( Ceramic & Induction) There is also information about assembly instructions on Conditional assembly instructions. Integrated Trinity can accept a bam file containing genome-aligned rna-seq reads as input. Note: on trinity board v1.0 we had to enlarge the holes of RGB LED by drilling, so the through hole connections might got lost! Match the front plate so all knobs and buttons go through and can be operated easily. Owners manuals or product information may not be available for all products indefinitely or at all, and may be permanently discontinued at the Companys discretion. For more precise control, the --bflyCPU option is available. StringTie is a fast and highly efficient assembler of RNA-Seq alignments into potential transcripts. View all Ovens, Compact 45 cm likeso:!! Version 1.2 solved problem with securing battery clip against ripping cable from board with simple solution. Bamboo can be maintained with mineral oil for best results. Phase 2: Assembling Clusters of Reads: this is the Butterfly/Parafly stage. Fix the knobs so they don't touch the plate while you tweak them. We believe that the miracle working church of Jesus Christ seen in the book of Acts is the blueprint for the church today! View All Microwaves, Model Number * 3-step process on how to find TRINITY products on BILT. Adobe Reader is necessary to view these files. Parts can also be requested online at www.trinityii.com (Help & More, Contact Us). TEAMSPORT Weight Bench Instruction Manual, KIMODIRECT Halterbank Weight Bench Instruction Manual, Parrot Uncle BBCPD1725A2 66 Inch Black LED Indoor Ceiling Fan User Guide, NORTH COAST ROCKETRY 07703 Launch Master Flying Model Rocket Launch Controller Instruction Manual, oricom OBHAD200 Nursery Calm Aroma Diffuser User Guide, Simer 3075SS Automatic Booster Pump Owners Manual, lxnav LX MOP2 Means of Propulsion Sensor 2 Installation Guide, havit SMART26 26 Keys Bluetooth Number Pad User Manual, EMERIL LAGASSE FAFO-001 French Door Air Fryer 360 Owners Manual, 10Gtek WD-4503AC Wireless Adapter Installation Guide, THETFORD SANICON Turbo 700 Owners Manual, FLOOR POLICE 15262-6 Cordless Electric Spinning Microfiber Flat Mop Instruction Manual, Weight capacity of Bench Top (evenly distributed), Weight capacity per shelf (evenly distributed), Total weight capacity of Shoe Bench (evenly distributed). Make sure knobs are soldered straight and as close to the PCB at its construction allows. TRINITY Communication Link The communication with the UAV Trinity is ensured by two links: Q Base, a 915MHz flight data link. Bend the legs of the components slightly, turn the board upside down and solder each connection properly. A copy of your original purchase receipt must accompany your service request. All replaced parts and Products become the property of Trinity and must be returned to Trinity. 3) This library does not provide owners manuals and product information for all of the products sold by the Company. Induction Trinity combines three independent software modules: Inchworm, Chrysalis, and Butterfly, applied sequentially to process large volumes of RNA-Seq reads. Trinity Assembly By bastl-instruments in Circuits Soldering 28,640 116 2 Featured Download Favorite By bastl-instruments Bastl Instruments Follow More by the author: Hey dudes!! On the audio connector try to solder it the way that there is NO gap between the connector and the PCB. It is advised that End User review and strictly adhere to these Cautions. He is passionate about evolution and the science behind the genome. First, lets solder all components on the component side of the PCB. He did a Bioinformatics Postdoc in Soybean genetics and now runs the Genome Informatics Facility at Iowa State University. Slide-Out very useful manuals finding browser extension. Than screw it. When you combine samples for de-novo transcriptome assembly with Trinity, do you suggest limiting the number of reads for each sample? The following details the steps involved in: Generating a Trinity de novo RNA-Seq assembly. This instructable is now updated for both versions 1.0 and 1.1. Double 60cm Make sure they are straight, otherwise you might have problems putting them through the housing. Reads are partitioned into coverage groups along the reference genome and each read cluster is assembled using the standard Trinity de novo assembly. Trinity Sequence Assembly Manual. 9 years ago Generating a Trinity de novo RNA-Seq assembly Evaluating the quality of the assembly Quantifying transcript expression levels Identifying differentially expressed (DE) transcripts Functionally annotating transcripts using Trinotate and predicting coding regions using TransDecoder Examining functional enrichments for DE transcripts using GOseq Filter Options Network monitoring and troubleshooting is hard. 4) Contents of owners manuals and content as found on korg.com may differ. IT IS NOT INTENDED FOR THE CONSUMER/END-USER. Rotate your device and follow the step-by-step assembly instructions. 7) Contacts for questions regarding the product, as well as other addresses, phone numbers, or email addresses listed in the owners manuals are current as of the date the manual was produced. You should know that there are some differences between some of the versions. Please tell us which model you purchased, the date of the purchase, and the problem with your Product. If you make some mods or tweaks, feel free to send us photos at info@bastl-instruments.com! STEP 2: Using the included HEX KEY (F), fasten all SCREWS (D) to connect SIDE FRAME (A) with BENCH TOP (B). Persons assembling the Trinity have to wear antistatic shoes. Aims. TRINITY model numbers usually start with: TBF, THA, TLS, TSN, & TXK and are then followed by a 4-5 digit number 2. TRINITY Customer Service provides the following replacement parts: Part Number Description1) PGR-03-081-3413 Bench Top (Gray)2) PRA-03-081-3413 Bench Top (Bronze Anthracite)3) PGR-14-023-1318 Side Frame (Gray)4) PRA-14-023-1318 Side Frame (Bronze Anthracite)5) ZBK-01-002-0635 Screw6) XBK-98-006-2525 Feet leveler7) PGR-03-082-3413 Shelf (Gray)8) PRA-03-082-3413 Shelf (Bronze Anthracite)9) XXX-97-001-0001 Hex Key. View All Hobs, Wall Canopy For soldering LEDs on SQNCR see the next step! Ceramic You can also watch these videos on our Youtube channel. Read the color stripes or measure the values with multimeter. . If you are missing any parts, need assistance with assembly or have questions, please contact TRINITY Customer Service: 800.985.5506 or customerservice@trinityii.com. Riftek RF096-35/55-100 Series User Manual Operation & user's manual (34 pages) LEGRAND 035 86 Installation . For the latest contact information, please refer to the Korg.com website. Comments Agree to privacy policy I wish to signup to the TRINITY Appliances newsletter for useful updates GitHub - trinityrnaseq/trinityrnaseq: Trinity RNA-Seq de novo transcriptome assembly trinityrnaseq / trinityrnaseq Public master 6 branches 40 tags Code brianjohnhaas update for latest dexseq formatting 57a58de 8 days ago 1,744 commits Analysis update for latest dexseq formatting 8 days ago Butterfly I wish to signup to the TRINITY Appliances newsletter for useful updates. Avoid harsh, abrasive cleaners, and other corrosive chemicals. Put the components and bend their legs slightly. Trinity, developed at the Broad Institute and the Hebrew University of Jerusalem, represents a novel method for the efficient and robust de novo reconstruction of transcriptomes from RNA-seq data. Identify the root-cause of network issues anywhere on your network. 1 Version 9 /2015 CONTENTS SELECTING THE CORRECT FRAME SIZE All the other components go now on the board. Check polarity indicated by the stripe diode. Name Transcriptome Assembly and Funtional Annotation; Description: This page describes a serie of tools and linux commands used to manipulate fastq files for transcriptome assembly and funtional annotation of transcrits using Trinity and Trinotate. SC26-4940-06 This chapter describes, in detail, the syntax and usage rules of each assembler instruction. This manual is intended to describe what you need to know to use gnu as. As you can see on picture, you have to put wire through holes dedicated to secure cables, before you solder them to board. Now connect speaker or headphones to check that everything works! - 4x 220R red red brown (on board v1.1 three of these 220R are labeled zOhm), - 1x 4.9K yellow white white brown (4.7k also works yellow violet red). View All Packages, Integrated The component side is ready so lets solder from the Interface side. If you made it so far, congratulations ! It uses a novel network flow algorithm as well as an optional de novo assembly step to assemble and quantitate full-length transcripts representing multiple splice variants for each gene locus. Definitely recommended! Red is + (positive) and black is - (negative). On the chip socket mind the polarity of chip indicated by the notch. Do not store the finished parts in the transport cases before, to avoid deposition of CA fumes on surfaces. Challenge Level: Requires some working knowledge of Linux, and determination. The volume knob might also need a bit of adjustment so it fits the holes (only for version 1.0). !tar-zxvfrnaseq_workshop_data.tgz! Trinity Assembly lasted ~10 times longer although it had more resources available. Examining functional enrichments for DE transcripts . View All Fridges, Wine Coolers Trinitys obligation under this warranty shall be limited to repair or replacement of, or adequate compensation for the Product which shall not be greater than the amount of the purchase price of the Product, at the option of Trinity, during the warranty period. It is easier to put a bit of solder on the cable before you put it through the holes. To know if Trinity is the right choice for your research, If one of your little LEDs doesn't work try to remake the traces as on the pictures. on Introduction, Check out our playlist showcasing possibilities of Trinity: http://bit.ly/1s97X4z, Make Your Own Customisable Desktop LED Neon Signs / Lights, Smart Light Conversion Using ESP8266 and a Relay, Wi-Fi Control of a Motor With Quadrature Feedback. Coronavirus Info. Curved Glass Wall Canopy This limited warranty shall not extend to anyone other than the original purchaser of this product, is nontransferable and states your exclusive remedy. NOTE: on version 1.1 there is extra hole for holding the battery clip and plastic piece. Trinity (and its employees, officers, members, managers, affiliates an assigns) shall not be liable for any incidental, consequential, special, indirect, remote, special or punitive damages for breach of any warranty, express or implied, including, but not limited to, lost profits, lost savings, loss of anticipated benefits and attorneys fees, which arise out of the purchase, use or inability to use the Product, whether arising out of contract, negligence, strict tort, product liability, or any other legal theory on which a claim is based. Found on the rating plate of your appliance. Identifying differentially expressed (DE) transcripts. 1) All intellectual property contained in this library - including owners manuals and product literature - is the sole property of KORG Inc. Trinity, developed at the Broad Institute and the Hebrew University of Jerusalem, represents a novel method for the efficient and robust de novo reconstruction of transcriptomes from RNA-seq data. These videos are available on each product pages. Place and solder the buttons. Please feel free to contact us. This manual also describes some of the machine-dependent features of various avors of the assembler. Free-standing Trinity Highway QuadGuard M10 Assembly Manual Assembly manual (57 pages) Trinity Highway CASS TL3: Frequently viewed Manuals. Some Trinity versions (SQNCR and DRUM) do have additional buttons at each side of bottom part of board (check last picture). The RGB LED goes as deep as the stoppers on its legs are. The official download page is here. 6) Please be aware that this service may be modified or terminated at any time by the Company without specific notice. SQNCR has more buttons and 3 small LEDs instead of the RGB LED and therefore has different resistors too. Please email or call. Coin . Include a copy of your original receipt with your submission. The assembly from start to finish can take anywhere from ~1/2 hour to 1 hour per million reads (your mileage may vary). Great extension! Now you can put in the felt protector and a 9V battery if you have. Here are main new features of the Trinity ViolinCase2: 1. Some states do not allow the exclusion or limitation of incidental, consequential, special, or punitive damages, so the above limitation or exclusion may not apply to you. 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trinity assembler manual